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Both aqueous mobile phase portions were pH latanoprost eye drops adjusted to 3.08. The mobile phase used for the tissue samples was 30 mM acetate/citrate fioricet tablets buffer with 5 mM octanesulfonic acid-acetonitrile (99:1, v/v). Determination of Acyclovir / Aciclovir in maternal plasma, amniotic fluid, fetal and placental tissues by high-performance latanoprost eye drops liquid / Aciclovir [9-[(2-hydroxyethoxy)-methyl]-guanosine, Zovirax, ACV] is a synthetic purine nucleoside analog active against herpes simplex virus types 1 (HSV-1), 2 (HSV-2), pain relief medication and varicella zoster virus. The assay was validated for each matrix over a range of 0.25-100 microg/ml over 3 days using five replicates of three spiked concentrations. Acyclovir / Aciclovir has frequently been used in HSV-2 seropositive mothers hair loss drugs to prevent prenatal transmission of herpes virus to their unborn children. Tissue samples were homogenized in distilled water, centrifuged, and extracted using a C(18) solid-phase tavanic extraction method prior to analysis. Baseline resolution was achieved for Acyclovir / Aciclovir and the internal standard gancyclovir, an anti-viral of similar structure to Acyclovir / Aciclovir, using an Agilent Eclipse XDB C(8) column (150 x 2.1 mm, 5 microm). Plasma and amniotic fluid samples were prepared by protein precipitation using 2 M perchloric acid and syringe filtering. The mobile phase used for the plasma and amniotic fluid was 10 mM acetate/citrate buffer-3.7 mM aqueous octanesulfonic acid (87.5:12.5, v/v) at a flow-rate of 0.2 ml/min. All separations were done using an Agilent 1100 Series HPLC system with UV detection of 254 nm. The relative standard deviation and percent error for each validation data set was 80%.. A fast and reproducible HPLC method for the determination of the highly polar acyclvoir in maternal rat plasma, amniotic fluid, placental tissue, and fetal tissue has been developed and validated.
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